Protein Purification From Molecular Mechanisms to Large-Scale Processes
by Ladisch, Michael R.; Builder, Stuart E.; Painton, Chih-duen C.; Willson, Richard C.Format: Hardcover
Pub. Date: 1990-05-05
Publisher(s): American Chemical Society
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Summary
This practical volume gives real-life examples of how separation techniques are used in industry with recombinant proteins and emphasizes which techniques might be more useful than others. An introductory chapter ties together the various topics and associates the methods used with examples of large-scale methodologies. The emphasis of the volume is on how series can be applied to developing strategies and appropriate approaches for difficult protein separation problems. This valuable book provides an update on some of the existing technology used in industry and presents separation examples of several commercially important and recently developed proteins.
Table of Contents
| Large-Scale Protein Purification: Introduction | |
| Strategies for Large-Scale Protein Purification | |
| Separations in Biotechnology: The Key Role of Adsorptive Separations | |
| Peptide and Protein Partitioning in Aqueous Two-Phase Systems: Effect of Amino Acid Sequence | |
| Protein Separation via Polyelectrolyte Complexation | |
| Mechanisms of Protein Retention in Hydrophobic Interaction Chromatography | |
| Anion Exchange Stationary Phase for b-Galactosidase, Bovine Serum Albumin, and Insulin: Separation and Sorption Characteristics | |
| Radial-Flow Affinity Chromatography for Trypsin Purification | |
| Impact of Continuous Affinity-Recycle Extraction (CARE) in Downstream Processing | |
| Novel Metal-Affinity Protein Separations | |
| Recovery of Recombinant Proteins by Immunoaffinity Chromatography | |
| Chelating Peptide-Immobilized Metal-Ion Affinity Chromatography | |
| Site-Specific Proteolysis of Fusion Proteins | |
| Purification Alternatives for IgM (Human) Monoclonal Antibodies | |
| Analytical, Preparative, and Large-Scale Zone Electrophoresis | |
| Applied Electric Fields for Downstream Processing | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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